IAA Research Platform

Product Code: imFlux-IAA-100

Part of the NMT BioFunction Research Platform series. The imFluxes-IAA-100 is used for testing living organisms’ physiological functions, and it is designed specifically for testing IAA, a class of plant hormones.

While maintaining the integrity of the samples, it can detect IAA influx and efflux and has sensitivity as high as femto (10-15) moles • cm-2 • s-1.

Currently, imFluxes-IAA-100 is the only real-time and dynamic information system for testing IAA in the world.

This platform would be beneficial for research in areas such as growth regulation, polar transport and herbicide mode-of-action

 

 

  NMT Reveals Functions of IAA in Potential Cancer Treatment

In-situ and non-invasive test on living samples

Provides information of samples in their normal physiological condition without damaging the samples

Real-time Testing

Can test and provide real-time data in as little as 5 seconds

Ionic and molecular measurements

This platform measures: IAA

Additional sensor options: none

(Other NMT BioFunction Research Platforms are able to measure Ca2+, H+, K+, Na+, Cl-, Mg2+, Cd2+, NH4+, NO3-, O2, H2O2, and/or IAA)

Long-term measurements

Can provide real-time and dynamic detection for many hours

No labeling

Without using the radioactive, chemical, pharmacological or other labeling methods, imFluxes-IAA-100 allows researchers to detect the tested ions and molecules in advance in a safe and environmentally friendly manner.

High resolution and wide range of sample sizes

Temporal resolution: 3s (response time) and 5s (measurement time);

Spatial resolution of ions: 1µm- 10µm;

Spatial resolution of molecules: 20µm—25µm;

Flux Sensitivity: 10-15mol•cm-2•s-1

No need to extract samples

Can detect samples directly without using grinding and other traditional extraction methods

Capable of testing various samples

Intact organisms, organs, tissues, cells, and even small condensed organelles can be tested

3D Flux Measurements

Different platforms in the NMT BioFunction Research Platform series can detect one-dimensional measurements (NMT BioFunction Research Platforms are the exception, for they can detect three-dimensional measurements).

After upgraded, all platforms in the series can detect three-dimensional measurements and provide flux information by measuring the concentration gradient in all x, y, and z directions. At the same time, sample positioning will become more precise and flexible, thus enhancing the repeatability of the experiment.

For more published works, see our Publications page.

 Research using NMT has been published in journals such as:

IAA research using NMT includes: 

- Yan S, et al. MeJA Affects Root Growth by Modulation of Transmembrane Auxin Flux in the Transition Zonepaper. J Plant Growth Regul, 2015. doi/10.1007/s00344-015-9530-9.

- Yan S, et al. Insect Herbivory of Leaves Affects the Auxin Flux Along Root Apices in Arabidopsis thaliana. Journal of Plant Growth Regulation. 2017. DOI: 10.1007/s00344-017-9688-4

- Wang WL,et al. The effect of polar auxin transport on adventitious branches formation in Gracilaria lichenoides in vitro. Physiologia Plantarum. 2016, doi: 10.1111/ppl.12464.

 

Motion Control Module ║ X-axis Motion Translation Stage
Load Capacity 156N
Travel distance 25.4mm
Mode Automatic
Control Stepper motor
Minimum step 39nm
Bidirectional repeatability 500nm
Motion Control Module ║ Y-axis Motion Translation Stage
Load Capacity 156N
Travel distance 25.4mm
Mode Manual
Control Lead screw drive
Motion Control Module ║ Z-axis Motion Translation Stage
Load Capacity 191N
Travel distance 25.4mm
Mode Manual
Control Lead screw drive
Motion Control Module ║ Lead screws
Screw Thread M1*0.25mm
Load Capacity 90N
Travel distance 25mm
Motion Control Module ║ Supporting Stage
Dimensions L200mm×W200mm×H5mm
Material-top M6 on 25mm
Adjustable supporting rod 150mm - 250mm
Signal Processing Module ║ Solid Reference Microelectrode
Temperature range -5°C - 100°C
Wire length 2 m
Tip diameter 2 mm
Signal Processing Module ║ Pre-amplifier (Polarographical Head Stages)
Type embedded current/voltage conversion
Resolution 1mV/pA
Signal Processing Module ║ Reference Microelectrode Holder
Rotation angle 360°
Linear Motion adjustable solid sliding rail
Signal Processing Module ║ Ground Wire Bracket
Size 80mm×20mm×30mm
Signal Processing Module ║ Data Acquisition System
Signal Acquisition mode 16 bits A/D conversion card
Operating system Windows7
Signal Processing Module ║ High-gain Main Amplifier for Molecule Sensor
Input voltage & frequency 100-240V AC 50/60 Hz
Amplifier gains ×100
Signal Processing Module ║ IAA Flux Sensor
Tip diameter 20µm
Sensor material Metal
Software ║ Non-invasive Ion & Molecule Flux Measurement Software V2.0
Manual English
Interface English/Chinese
Software module(s) IAA measurement module
Data output format MS Excel
Account Management Multi user
Supports Chinese input yes
Potential/Slope value over range alarm module yes
Data optimization module yes